DOI: 10.1101/2023.05.25.540669

In vivo long-term voltage imaging by genetically encoded voltage indicator reveals spatiotemporal dynamics of spinal cord neuronal populations during development

A.Shiraishi A. Hayashi N. Fukuda M. Hishinuma H. Miyazawa S. Tsuda

摘要

A central question of brain development lies in how individual neurons emerge and organize communities to acquire various functions. Voltage imaging provides unique approaches to address this by enabling simultaneous, non-invasive, in vivo recording of voltage dynamics from a population of cells. Recently, genetically encoded voltage indicators (GEVIs) facilitate cell-type specific imaging of voltage dynamics. However, it has not been applied to brain development. Here, we applied ArcLight, a GEVI utilizing voltage-sensitive domain, to zebrafish and established experimental approaches for analyzing voltage and morphology during development, focusing on the spinal cord and cerebellum. We initially demonstrated that Arclight was widely distributed in the neural tissues. By voltage imaging, we successfully visualized the coordinated, spontaneous activity of spinal cord neurons in their early stage of development at a high spatiotemporal resolution, at subcellular and population levels. Hyperpolarization and subthreshold signals were also detected. Finally, long-term voltage imaging during development revealed the process of changes in voltage dynamics in neuron populations, which were accompanied by axonal outgrowth. Voltage imaging could greatly contribute to our understanding of the functional organization of the nervous system during development.

展开全部

图表提取

论文十问

参考文献

被引用

提问与回答

暂无人提供速读十问回答

被引用

笔记

问答